Isolation of infectious bronchitis virus from broiler

Abstract

Infectious bronchitis virus (IBV) was isolated from broilers chicks. The virus isolation done by samples inoculation in chicken egg embryos via allontoic cavity route which showed sensitivity for virus isolation resulting in stunting and rounding of inoculated embryos, as well as thickening of chorioallantoic membrane (CAM). Cultivation of the isolated virus was carried out in the yolk sac and embryo vein .The virus was grown in chicken kidney cells (CKC) cultures resulting in cytopathic effect ( CPE ) after 24 h. post inoculation including swelling and accumulation of cells which increased in 2nd& 3rd day post inoculation. The diagnosis of isolated virus was carried out by Agar gel …………. precipitation test (AGPT) using reference positive antiserum and Serum neutralization test (SNT) using CKC tissue culture.