Development of serological assays to identify Helicobacter suis and H. pylori infections
Hidenori Matsui,
Emiko Rimbara,
Masato Suzuki,
Kengo Tokunaga,
Hidekazu Suzuki,
Masaya Sano,
Takashi Ueda,
Hitoshi Tsugawa,
Sohachi Nanjo,
Akira Takeda,
Makoto Sasaki,
Shuichi Terao,
Tsuyoshi Suda,
Sae Aoki,
Keigo Shibayama,
Hiroyoshi Ota,
Katsuhiro Mabe
Affiliations
Hidenori Matsui
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan; Department of Bacteriology, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan; Ōmura Satoshi Memorial Institute, Kitasato University, Tokyo 108-8641, Japan; Corresponding author
Emiko Rimbara
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan
Masato Suzuki
Antimicrobial Research Center, National Institute of Infectious Diseases, Tokyo 189-0002, Japan
Kengo Tokunaga
Department of General Medicine, Kyorin University School of Medicine, Tokyo 181-8611, Japan
Hidekazu Suzuki
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Japan
Masaya Sano
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Japan
Takashi Ueda
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Japan
Hitoshi Tsugawa
Division of Host Defense Mechanism, Tokai University School of Medicine, Kanagawa 259-1193, Japan
Sohachi Nanjo
Third Department of Internal Medicine, University of Toyama, Toyama 930-0194, Japan
Akira Takeda
Takeda Hospital, Fukuoka 822-0027, Japan
Makoto Sasaki
Division of Gastroenterology, Department of Internal Medicine, Aichi Medical University School of Medicine, Aichi 480-1103, Japan
Shuichi Terao
Department of Gastroenterology, Kakogawa Central City Hospital, Hyogo 675-8611, Japan
Tsuyoshi Suda
Department of Gastroenterology, Kanazawa Municipal Hospital, Ishikawa 921-8105, Japan
Sae Aoki
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo 208-0011, Japan
Keigo Shibayama
Department of Bacteriology, Nagoya University Graduate School of Medicine, Aichi 466-8550, Japan
Hiroyoshi Ota
Department of Biomedical Laboratory Sciences, Shinshu University School of Medicine, Nagano 390-8621, Japan
Katsuhiro Mabe
Junpukai Health Maintenance Center Kurashiki, Okayama 712-8014, Japan; Corresponding author
Summary: Helicobacter suis, hosted by hogs, is the most prevalent gastric non-Helicobacter pylori Helicobacter species found in humans. Recent studies have suggested that H. suis infection has caused many cases of gastric disease, but the transmission route from hogs remains unclear. Diagnostic methods based on H. suis urease activity often yield negative results, and there is no reliable method for diagnosing H. suis infection in clinical practice without gastric biopsy specimens. This study presents the world’s first use of whole-bacterial cell ELISA to simultaneously assess H. suis and H. pylori infections. The ELISAs showed high accuracy, with an area under the ROC curve of 0.96, 100% sensitivity, 92.6% specificity, 76.9% positive predictive value, and 100% negative predictive value for the H. suis test, and an area under the ROC curve of 0.92, 88.2% sensitivity, 87.5% specificity, 65.2% positive predictive value, and 96.6% negative predictive value for the H. pylori test.
