Frontiers in Plant Science (Apr 2023)
The apple 14-3-3 gene MdGRF6 negatively regulates salt tolerance
- Yuqing Zhu,
- Yuqing Zhu,
- Yuqing Zhu,
- Yuqing Zhu,
- Wei Kuang,
- Wei Kuang,
- Wei Kuang,
- Wei Kuang,
- Jun Leng,
- Jun Leng,
- Jun Leng,
- Jun Leng,
- Xue Wang,
- Xue Wang,
- Xue Wang,
- Xue Wang,
- Linlin Qiu,
- Linlin Qiu,
- Linlin Qiu,
- Linlin Qiu,
- Xiangyue Kong,
- Xiangyue Kong,
- Xiangyue Kong,
- Xiangyue Kong,
- Yongzhang Wang,
- Yongzhang Wang,
- Yongzhang Wang,
- Yongzhang Wang,
- Qiang Zhao,
- Qiang Zhao,
- Qiang Zhao,
- Qiang Zhao
Affiliations
- Yuqing Zhu
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Yuqing Zhu
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Yuqing Zhu
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Yuqing Zhu
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Wei Kuang
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Wei Kuang
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Wei Kuang
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Wei Kuang
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Jun Leng
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Jun Leng
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Jun Leng
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Jun Leng
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Xue Wang
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Xue Wang
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Xue Wang
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Xue Wang
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Linlin Qiu
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Linlin Qiu
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Linlin Qiu
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Linlin Qiu
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Xiangyue Kong
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Xiangyue Kong
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Xiangyue Kong
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Xiangyue Kong
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Yongzhang Wang
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Yongzhang Wang
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Yongzhang Wang
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Yongzhang Wang
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- Qiang Zhao
- College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong, China
- Qiang Zhao
- Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Qingdao Agricultural University, Qingdao, Shandong, China
- Qiang Zhao
- Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao Agricultural University, Qingdao, Shandong, China
- Qiang Zhao
- Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, Qingdao Agricultural University, Qingdao, Shandong, China
- DOI
- https://doi.org/10.3389/fpls.2023.1161539
- Journal volume & issue
-
Vol. 14
Abstract
The 14-3-3 (GRF, general regulatory factor) regulatory proteins are highly conserved and are widely distributed throughout the eukaryotes. They are involved in the growth and development of organisms via target protein interactions. Although many plant 14-3-3 proteins were identified in response to stresses, little is known about their involvement in salt tolerance in apples. In our study, nineteen apple 14-3-3 proteins were cloned and identified. The transcript levels of Md14-3-3 genes were either up or down-regulated in response to salinity treatments. Specifically, the transcript level of MdGRF6 (a member of the Md14-3-3 genes family) decreased due to salt stress treatment. The phenotypes of transgenic tobacco lines and wild-type (WT) did not affect plant growth under normal conditions. However, the germination rate and salt tolerance of transgenic tobacco was lower compared to the WT. Transgenic tobacco demonstrated decreased salt tolerance. The transgenic apple calli overexpressing MdGRF6 exhibited greater sensitivity to salt stress compared to the WT plants, whereas the MdGRF6-RNAi transgenic apple calli improved salt stress tolerance. Moreover, the salt stress-related genes (MdSOS2, MdSOS3, MdNHX1, MdATK2/3, MdCBL-1, MdMYB46, MdWRKY30, and MdHB-7) were more strongly down-regulated in MdGRF6-OE transgenic apple calli lines than in the WT when subjected to salt stress treatment. Taken together, these results provide new insights into the roles of 14-3-3 protein MdGRF6 in modulating salt responses in plants.
Keywords
