Transcriptome analysis reveals the high temperature induced damage is a significant factor affecting the osmotic function of gill tissue in Siberian sturgeon (Acipenser baerii)
Shiyong Yang,
Datian Li,
Langkun Feng,
Chaoyang Zhang,
Dandan Xi,
Hongli Liu,
Chaozhan Yan,
Zihan Xu,
Yujie Zhang,
Yunkun Li,
Taiming Yan,
Zhi He,
Jiayun Wu,
Quan Gong,
Jun Du,
Xiaoli Huang,
Xiaogang Du
Affiliations
Shiyong Yang
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Datian Li
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Langkun Feng
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Chaoyang Zhang
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Dandan Xi
College of Landscape Architecture, Sichuan Agricultural University
Hongli Liu
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Chaozhan Yan
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Zihan Xu
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Yujie Zhang
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Yunkun Li
College of Life Science, Sichuan Agricultural University
Taiming Yan
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Zhi He
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Jiayun Wu
College of Life Science, Sichuan Agricultural University
Quan Gong
Fisheries Institute, Sichuan Academy of Agricultural Sciences
Jun Du
Fisheries Institute, Sichuan Academy of Agricultural Sciences
Xiaoli Huang
Department of Aquaculture, College of Animal Science & Technology, Sichuan Agricultural University
Xiaogang Du
College of Life Science, Sichuan Agricultural University
Abstract Background Maintaining osmotic equilibrium plays an important role in the survival of cold-water fishes. Heat stress has been proven to reduce the activity of Na+/K+-ATPase in the gill tissue, leading to destruction of the osmotic equilibrium. However, the mechanism of megatemperature affecting gill osmoregulation has not been fully elucidated. Results In this study, Siberian sturgeon (Acipenser baerii) was used to analyze histopathological change, plasma ion level, and transcriptome of gill tissue subjected to 20℃, 24℃and 28℃. The results showed that ROS level and damage were increased in gill tissue with the increasing of heat stress temperature. Plasma Cl− level at 28℃ was distinctly lower than that at 20℃ and 24℃, while no significant difference was found in Na+ and K+ ion levels among different groups. Transcriptome analysis displayed that osmoregulation-, DNA-repair- and apoptosis-related terms or pathways were enriched in GO and KEGG analysis. Moreover, 194 osmoregulation-related genes were identified. Amongst, the expression of genes limiting ion outflow, occluding (OCLN), and ion absorption, solute carrier family 4, member 2 (AE2) solute carrier family 9, member 3 (NHE3) chloride channel 2 (CLC-2) were increased, while Na+/K+-ATPase alpha (NKA-a) expression was decreased after heat stress. Conclusions This study reveals for the first time that the effect of heat stress on damage and osmotic regulation in gill tissue of cold-water fishes. Heat stress increases the permeability of fish’s gill tissue, and induces the gill tissue to keep ion balance through active ion absorption and passive ion outflow. Our study will contribute to research of global-warming-caused effects on cold-water fishes.
