Evaluation of a novel urinary antigen test kit for diagnosing Legionella pneumonia

Akihiro Ito; Yoshihiro Yamamoto; Yoshikazu Ishii; Akihito Okazaki; Yoshihisa Ishiura; Yukio Kawagishi; Yasuo Takiguchi; Kazuma Kishi; Yoshio Taguchi; Takashi Shinzato; Yasumi Okochi; Ryuji Hayashi; Yoshitaka Nakamori; Yoshiko Kichikawa; Kengo Murata; Hiroaki Takeda; Futoshi Higa; Takayuki Miyara; Keisuke Saito; Takeo Ishikawa; Tadashi Ishida; Kazuhiro Tateda

International Journal of Infectious Diseases (Feb 2021)

Evaluation of a novel urinary antigen test kit for diagnosing Legionella pneumonia

Akihiro Ito,
Yoshihiro Yamamoto,
Yoshikazu Ishii,
Akihito Okazaki,
Yoshihisa Ishiura,
Yukio Kawagishi,
Yasuo Takiguchi,
Kazuma Kishi,
Yoshio Taguchi,
Takashi Shinzato,
Yasumi Okochi,
Ryuji Hayashi,
Yoshitaka Nakamori,
Yoshiko Kichikawa,
Kengo Murata,
Hiroaki Takeda,
Futoshi Higa,
Takayuki Miyara,
Keisuke Saito,
Takeo Ishikawa,
Tadashi Ishida,
Kazuhiro Tateda

Affiliations

Akihiro Ito: Department of Respiratory Medicine, Ohara Healthcare Foundation, Kurashiki Central Hospital, 1-1-1 Miwa, Kurashiki, Okayama 710-8602, Japan; Corresponding author.
Yoshihiro Yamamoto: Department of Clinical Infectious Diseases, Toyama University Hospital, 2630 Sugitani, Toyama, Toyama 930-0194, Japan
Yoshikazu Ishii: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan
Akihito Okazaki: Department of Respiratory Medicine, Koseiren Takaoka Hospital, 5-10 Eirakumachi, Takaoka, Toyama 933-8555, Japan
Yoshihisa Ishiura: First Department of Internal Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka 570-8507, Japan
Yukio Kawagishi: Department of Internal Medicine, Kurobe City Hospital, 1108-1 Mikkaichi, Kurobe, Toyama 938-8502, Japan
Yasuo Takiguchi: Department of Respiratory Medicine, Chiba Aoba Municipal Hospital, 1273-2 Aoba-chou, Chuo-ku, Chiba, Chiba 260-0852, Japan
Kazuma Kishi: Department of Respiratory Medicine, Toho University Omori Medical Center, 6-11-1 Omori-nishi, Ota-ku, Tokyo 143-8541, Japan
Yoshio Taguchi: Department of Respiratory Medicine, Tenri Hospital, 200 Mishima-cho, Tenri, Nara 632-8552, Japan
Takashi Shinzato: Department of Infectious Diseases and Internal Medicine, Nakagami General Hospital, 610 Noborikawa, Okinawa, Okinawa 904-2195, Japan
Yasumi Okochi: Department of Respiratory Medicine, Japan Community Health Care Organization, Tokyo Yamate Medical Center, 3-22-1 Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan
Ryuji Hayashi: Clinical Oncology, Toyama University Hospital, 2630 Sugitani, Toyama, Toyama 930-0194, Japan
Yoshitaka Nakamori: Division of Respiratory Medicine, Mishuku Hospital, 5-33-12 Kamimeguro, Meguro-ku, Tokyo 153-0051, Japan
Yoshiko Kichikawa: Division of Respiratory Medicine, Mishuku Hospital, 5-33-12 Kamimeguro, Meguro-ku, Tokyo 153-0051, Japan
Kengo Murata: Department of Respiratory Medicine, Tokyo Metropolitan Tama Medical Center, 2-8-29 Musashidai, Fuchu, Tokyo 183-8524, Japan
Hiroaki Takeda: Department of Respiratory Medicine, Yamagata Saisei Hospital, 79-1 Okimachi, Yamagata, Yamagata 990-8545, Japan
Futoshi Higa: Department of Internal Medicine, National Hospital Organization Okinawa Hospital, 3-20-14 Ganeko, Ginowan, Okinawa 901-2214, Japan
Takayuki Miyara: Department of Infection Prevention and Control, Kobe University Hospital, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe, Hyogo 650-0017, Japan
Keisuke Saito: Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University School of Medicine, 3-19-18 Nishi-shinbashi, Minato-ku, Tokyo, Japan
Takeo Ishikawa: Division of Respiratory Diseases, Department of Internal Medicine, The Jikei University Daisan Hospital, 4-11-1 Izumihoncho, Komae, Tokyo 201-8601, Japan
Tadashi Ishida: Department of Respiratory Medicine, Ohara Healthcare Foundation, Kurashiki Central Hospital, 1-1-1 Miwa, Kurashiki, Okayama 710-8602, Japan
Kazuhiro Tateda: Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540, Japan

Journal volume & issue: Vol. 103
pp. 42 – 47

Abstract

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Objectives: The aim of this study was to evaluate the diagnostic utility of a novel test kit that could theoretically detect all serogroups of Legionella pneumophila for diagnosing Legionella pneumonia, in comparison with existing kits. Methods: This study was conducted in 16 hospitals in Japan from April 2016 to December 2018. Three urinary antigen test kits were used: the novel kit (LAC-116), BinaxNOW Legionella (Binax), and Q-line Kyokutou Legionella (Q-line). In addition, sputum culture and nucleic acid detection tests and serum antibody tests were performed where possible. The diagnostic accuracy and correlations of the novel kit with the two existing kits were analyzed. Results: In total, 56 patients were diagnosed with Legionella pneumonia. The sensitivities of LAC-116, Binax, and Q-line were 79%, 84%, and 71%, respectively. The overall match rate between LAC-116 and Binax was 96.8% and between LAC-116 and Q-line was 96.4%. One patient had L. pneumophila serogroup 2, and only LAC-116 showed a positive result, whereas Binax and Q-line did not. Conclusions: The novel Legionella urinary antigen test kit was useful for diagnosing Legionella pneumonia. In addition, it could detect Legionella pneumonia caused by non-L. pneumophila serogroup 1.

Published in International Journal of Infectious Diseases

ISSN: 1201-9712 (Print); 1878-3511 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: http://www.journals.elsevier.com/international-journal-of-infectious-diseases/

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