Effect of Serum Albumin on Porphyrin-Quantum Dot Complex Formation, Characteristics and Spectroscopic Analysis
André L. S. Pavanelli,
Leandro N. C. Máximo,
Roberto S. da Silva,
Iouri E. Borissevitch
Affiliations
André L. S. Pavanelli
Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-900, Brazil
Leandro N. C. Máximo
Instituto Federal de Educação, Ciência e Tecnologia Goiano, Urutaí 75790-000, Brazil
Roberto S. da Silva
Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-900, Brazil
Iouri E. Borissevitch
Departamento de Física, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, Ribeirão Preto 14040-900, Brazil
The effect of bovine serum albumin (BSA) upon interaction between CdTe QD functionalized by 3-Mercaptopropionic Acid (CdTe-3-MPA QD) and two water soluble porphyrins: positively charged meso-tetra methyl pyridyl porphyrin (TMPyP) and negatively charged meso-tetrakis(p-sulfonato-phenyl) porphyrin (TPPS4), was studied in function of pH using the steady-state and time resolved optical absorption and fluorescence spectroscopies. It was shown that, depending on the charge state of the components, interaction with albumin could either prevent the formation of the QD…PPh complex, form a mixed QD…PPh…BSA complex or not affect PPh complexation with QD at all. The obtained results may be of interest for application in photomedicine.
