Systematic study of the employment of enzymatic catalysts on the formation of carbon-heteroatoms

Abstract

The classic thio-Michael reaction is the one that involves the addition of a sulfur atom like the nucleophile of the reaction to an electrophile α,β -insaturated. There are many reports in the literature which describe the need of the electrophile activation using, for instance, Lewis acid. However, there is a slope that aims this activation using biocatalysts such as enzymes. In this sense, this study aimed to analyze some enzymes on the thio-Michael reaction. Some biocatalysts were tested such as the enzymes like Lipase, Lipozye and Chymosine (Figure 1). The reactions results indicated that the application of these biocatalysts proportioned a shorter reaction time from one day to four hours with high yield levels.

Keywords

• biotransformation, enzymes, tia-michael