Preparation of Hyperimmune Sera to Thermoextracts of Brucella S- and L-Forms

Abstract

Objective of the study was to prepare hyperimmune sera to Brucella S- and L-form thermo-extracts. Materials and methods. Thermo-extracts from Brucella abortus I-206 strain in S- and L-forms were used. Chinchilla rabbits were used as animals, sera producers. Specific activity was determined by test-tube agglutination with Brucella L-form corpuscular experimental and commercial Brucella colored diagnosticums, while specificity – with heterologous strains: Francisella tularensis 15 NIIEG, Vibrio choleraе 5 (Ogawa), V. choleraе 35 А3 (Inaba), Yersinia enterocolitica О:9, Y. pestis EV NIIEG, Escherichia coli 3912/41. Protein content in the obtained extract was defined using Lowry method. Results and conclusions. Consequently, the antigen activity of the generated thermo-extracts has been shown, the immunization schemes have been developed permitting to obtain highly active and specific sera that could be used for identification and differentiation of brucellosis causative agent.

Keywords

• brucella
• thermo-extract
• s- and l-form
• sera