HISTOPATHOLOGY POST INTRAPERITONEAL INFECTION WITH Providencia rettgeri IN MICE

Abstract

The Providencia rettgeri (P.rettgeri) is an opportunistic pathogen of human and animals and has a clinical importance as a nosocomial pathogen causing infections in the urinary system after catheterization and in immunocompromised patients less than human feces, bile and sputum. To estimati the pathogenic role of P.rettgeri in mice post-infection intraperitonial infection (I/P). thirty mice from both sexes, aged 6-7 weeks and weight 25-30 gm. were infected I/P by a whole bacterial suspension of P.rettgeri 0.5 ml (containing 1x108 CFU/ml), the negative control group injected I/P with (0.5 ml) PBS,five mice were sacrificed after (24, 48hrs. and 4,7 and 14 days) post-inoculation, also (n=5) were considered as negative control group, for histopathological examination. The histopathological changes at 24,48hrs until 4 days; recorded mild to moderate acute inflammatory cells reaction, edema, fibrinous exudate. At 7&14 days there was also necrosis and focal aggregation of mononuclear cells. Strong pathogenic role of P.rettegri in induction acute inflammatory response in experimental lab mice.

Keywords

• rettgeri
• catheterization
• bile