Multienzymatic system encapsulation: Application to the lactoperoxidase system

Abstract

The use of enzyme often implicates a stabilization to limit loss of activity and a suitable dry formulation to simplify handling. Freeze drying and spray drying are two classic methods but when complex systems are studied, it becomes difficult to achieve a correct formulation. In this work, the realization of a dry microbead containing a multienzymatic system (2 enzymes, 2 substrates) is presented. The lactoperoxidase system associated with an enzymatic hydrogen peroxide generator was chosen as the model. The microbead, made by spray drying and spray coating, was characterized in terms of size, shape, chemical structure, flow ability, encapsulation efficiency and antimicrobial activity. Different compositions of the coat were tested, as well as their influence on the bead properties.

Keywords

• Enzymes
• Encapsulation
• Lactoperoxidase