Frontiers in Veterinary Science (Jan 2022)

Tylosin Inhibits Streptococcus suis Biofilm Formation by Interacting With the O-acetylserine (thiol)-lyase B CysM

  • Yonghui Zhou,
  • Yonghui Zhou,
  • Fei Yu,
  • Mo Chen,
  • Yuefeng Zhang,
  • Qianwei Qu,
  • Yanru Wei,
  • Chunmei Xie,
  • Tong Wu,
  • Yanyan Liu,
  • Zhiyun Zhang,
  • Xueying Chen,
  • Chunliu Dong,
  • Ruixiang Che,
  • Yanhua Li

DOI
https://doi.org/10.3389/fvets.2021.829899
Journal volume & issue
Vol. 8

Abstract

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Streptococcus suis (S. suis) can decrease its virulence or modify local conditions through biofilm formation, which promotes infection persistence in vivo. Biofilm formation is an important cause of chronic drug-resistant S. suis infection. The aim of this study was to evaluate whether tylosin effectively inhibits S. suis biofilm formation by interacting with O-acetylserine (thiol)-lyase B (CysM), a key enzymatic regulator of cysteine synthesis. Biofilm formation of the mutant (ΔcysM) strain was significantly lower compared to the wild-type ATCC 700794 strain. Tylosin inhibited cysM gene expression, decreased extracellular matrix contents, and reduced cysteine, homocysteine, and S-adenosylmethionine levels, indicating its potential value as an effective inhibitor of S. suis biofilm formation. Furthermore, using biolayer interferometry technology and fourier-transform infrared spectroscopy, we found that tylosin and CysM could be combined directly. Overall, these results provide evidence that tylosin inhibits S. suis biofilm formation by interacting with CysM.

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