Tricholoma matsutake polysaccharides suppress excessive melanogenesis via JNK-mediated pathway: Investigation in 8- methoxypsoralen induced B16–F10 melanoma cells and clinical study
Yang Yang,
Zheng Lv,
Quan An,
Detian Xu,
Longjie Sun,
Yiming Wang,
Xuexue Chen,
Xue Shao,
Tong Huo,
Shuangrui Yang,
Jiali Liu,
Haoshu Luo,
Qianghua Quan
Affiliations
Yang Yang
Yunnan Baiyao Group Co., Ltd., Kunming, 650504, China; East Asia Skin Health Research Center, Beijing, 100037, China
Zheng Lv
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China
Quan An
Yunnan Baiyao Group Co., Ltd., Kunming, 650504, China; East Asia Skin Health Research Center, Beijing, 100037, China
Detian Xu
Shanghai Skin Disease Hospital, Tongji University Medical School, Shanghai, 200050, China; The Ice Dermalab, Shanghai, 200050, China
Longjie Sun
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China
Yiming Wang
East Asia Skin Health Research Center, Beijing, 100037, China
Xuexue Chen
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China
Xue Shao
Yunnan Baiyao Group Co., Ltd., Kunming, 650504, China; East Asia Skin Health Research Center, Beijing, 100037, China
Tong Huo
Yunnan Baiyao Group Co., Ltd., Kunming, 650504, China; East Asia Skin Health Research Center, Beijing, 100037, China
Shuangrui Yang
Kunming Hospital of Traditional Chinese Medicine, Kunming, 650011, China
Jiali Liu
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China
Haoshu Luo
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China; Corresponding author. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
Qianghua Quan
Yunnan Baiyao Group Co., Ltd., Kunming, 650504, China; East Asia Skin Health Research Center, Beijing, 100037, China; Corresponding author. Yunnan Baiyao Group Co., Ltd., Kunming, 650000, China.
Skin hyperpigmentation is a worldwide condition associated with augmented melanogenesis. However, conventional therapies often entail various adverse effects. Here, we explore the safety range and depigmentary effects of polysaccharides extract of Tricholoma matsutake (PETM) in an in vitro model and further evaluated its efficacy at the clinical level. An induced-melanogenesis model was established by treating B16–F10 melanoma cells with 8-methoxypsoralen (8-MOP). Effects of PETM on cell viability and melanin content were examined and compared to a commonly used depigmentary agent, α-arbutin. Expressions of key melanogenic factors and upstream signaling pathway were analysed by quantitative PCR and western blot. Moreover, a placebo-controlled clinical study involving Chinese females with skin hyperpigmentation was conducted to measure the efficacy of PETM on improving facial pigmented spots, melanin index, and individual typology angle (ITA°). Results demonstrated that PETM (up to 0.5 mg/mL) had little effect on the viability and motility of B16–F10 cells. Notably, it significantly suppressed the melanin content and expressions of key melanogenic factors induced by 8-MOP in B16–F10 melanoma cells. Western blotting results revealed that PETM inhibited melanogenesis by inactivating c-Jun N-terminal kinase (JNK), and this inhibitory role could be rescued by JNK agonist treatment. Clinical findings showed that PETM treatment resulted in a significant reduction of facial hyperpigmented spot, decreased melanin index, and improved ITA° value compared to the placebo-control group. In conclusion, these in vitro and clinical evidence demonstrated the safety and depigmentary efficacy of PETM, a novel polysaccharide agent. The distinct mechanism of action of PETM on melanogenic signaling pathway positions it as a promising agent for developing alternative therapies.
