Isolation, Identification and Characterization of Novel Azo Dye Degrading Bacteria from the Industrial Effluents of Raipur City, Chhattisgarh

Abstract

Various chromophores are used to make our day-to-day life colorful. Dyes that are used at a large scale are made using these chromophores. The dyes, especially azo dyes are recalcitrant to the degradation due to the presence of aromatic rings in their structure. Several methods have been developed to reduce the harmful impacts of these dyes on the environment. However, none of the processes is safe and fully effective. In this study, we used bacteria as a bioremediation agent and optimized the various parameters for the bacteria to degrade the dye at its maximum ability. It was found that the isolated bacteria were Aneurinibacillus sp. and it completely decolorized methyl orange at a concentration of 20 mg.L-1 after 4 days of incubation. The optimum pH for the functioning of bacteria was 5 and the activity decreased as the pH increased. It was also observed that the addition of glucose and yeast extract increased the dye degradation significantly.

Keywords

• aneurinibacillus, azo-dye degradation, bioremediation, dye decolorization, methyl orange