The Influence of Supplementation of Feed with Lactobacillus reuteri L2/6 Biocenol on Intestinal Microbiota of Conventional Mice

Abstract

FISH (fluorescence in situ hybridization) analysis of the intestinal tract of conventional mice, following 14-day supplementation of feed with host non-specific (porcine) strain L. reuteri L2/6, showed in the presence of complex microbiota, a significant increase in the counts of representatives of the genera Lactobacillus and Bifidobacterium, and a significant decrease in the representatives of the genera Clostridium, Bacteroides and Enterobacteriaceae. At the same time, the supplemented strain stimulated the population of caecal lactobacilli of the species L. reuteri. These results demonstrated that the L. reuteri L2/6 colonised the jejunum, ileum and caecum and modulated the investigated intestinal microbiota.

Keywords

• fish analysis
• intestinal microbiota
• lactobacillus reuteri
• probiotics