EFFECT OF PLANT GROWTH REGULATORS AND EXPLANTS ON THE MICROPROPAGATION OF CAPPARIS SPINOSA L.

Abstract

This research sought to propagate caper plants in vitro through the cultivation of explants taken from seedlings derived from in vitro tissue culture and their subsequent cultivation in the Murashige and Skoog (MS) medium.The results show that at the initiation stage, the best percentage of dead explants was at a concentration of 1.5 mg L-1 when the shoot tip was treated with benzyladenine, reaching 5%. In the same treatment, the highest percentage of growth-responsive explants reached 80%. The results at the multiplication stage show that shoot tips treated with the regulator BA at a concentration of 1.5 mg L-1 may be superior in terms of the number of leaves, branch length, and number of branches, reaching 7.30 leaf plantlets-1, 3.78 cm, and 3.50 branch plantlets-1, respectively.The results further demonstrate a statistically significant impact of kinetin on the multiplication of the node in the studied traits at a concentration of 2 mg L-1, recording 6.80 leaf plantlets-1, 3.95 cm, and 2.20 branch plantlets-1. As for the rooting stage, of the tested concentrations, 0.5 mg L-1 IBA resulted in the highest rooting percentage, reaching 80% in a medium supplemented with half of the salt concentrations. The concentration of 1 mg L-1 achieved the highest average for the root length trait at 3.70 cm, and an average root number of 2.91 per plantlet-1.

Keywords

• capparis spinosa
• in vitro
• benzyladenine
• kinetin