Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle

Abstract

Summary: We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload.For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022a). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Microscopy
• Model Organisms
• Antibody
• Stem Cells