Zhongguo quanke yixue (Jan 2024)
Effect of Simiao Decoction on Vitamin D System and Neutrophil Extracellular Traps in Rats with Rheumatoid Arthritis
Abstract
Background As China enters the period of accelerated development of population aging, the proportion of rheumatoid arthritis cases is increasing, causing a serious burden on society and families. The active ingredients in simiao decoction have improved effect, but there is a lack of relevant studies to analyze the action and mechanism of prescriptions. Objective To observe the intervention effect of simiao decoction on rats with rheumatoid arthritis established by collageninduced arthritis and its effect on vitamin D system and neutrophil extracellular traps (NETs) . Methods From November 2021 to January 2023, 70 SD female rats were selected. The rats were randomly divided into the blank group (control group), model group (model group), methotrexate group (MTX group), vitamin D group (VD group), Simiao decoction low-dose group (SMT-L group), Simiao decoction medium-dose group (SMT-M group) and Simiao decoction high-dose group (SMT-H group) by random number table method, with 10 rats in each group. The rat model of synovial arthritis was established by collagen induction method and the gavage treatment was started 7 d after modeling. The degree of ankle swelling was assessed, and the arthritis index (AI) score of rats in each group the levels of interleukin 6 (IL-6), 25-hydroxyvitamin D3 [25 (OH) D3], NETs in serum of rats and the expression levels of 24-hydroxylase (CYP24A1), 1-α hydroxylase (CYP27B1), MPO, vitamin D receptor (VDR) and elastase (NE) in synovial tissue of joint were measured. Results The degree of ankle swelling in the control, MTX, VD, SMT-L, SMT-M and SMT-H groups was lower than that in the model group on day 7, the degree of ankle swelling in the model group was higher than the control group, the SMT-M group was higher than the model group on day 14, the degree of ankle swelling in the model group was higher than the control and MTX groups and lower than the VD, SMT-L, SMT-M and SMT-H groups on day 21, the degree of ankle swelling in the control, MTX, VD, SMT-L, SMT-M and SMT-H groups was higher than the model group, and the difference was statistically significant (P<0.05). The AI score of rats in the SMT-H group was lower than that in the model group on day 14, the AI score in the MTX, VD, SMT-L and SMT-H groups was lower than that in the model group on day 21, the score of rats in the MTX, VD, SMT-L, SMT-M, and SMT-H groups was lower than that in the model group on day 28, the difference was statistically significant (P<0.05). Immunohistochemical results showed significant differences in the expression levels of MPO and TNF-α in synovial tissue among the 7 groups (P<0.05), MPO and TNF-α in the model group were higher than those in the control group, while MPO and TNF-α in the MTX, VD and SMT-H groups were lower than those in the model group, TNF-α in the SMT-M group was lower than that in the model group, and the difference was statistically significant (P<0.05). IL-6 and NETs in the model group were higher than those in the control group, and 25 (OH) D3 was lower than that in the control group. IL-6 and NETs in the MTX, VD and SMT-H groups were lower than those in the model group, and 25 (OH) D3 was higher than that in the model group. NETs in the SMT-M group were lower than those in the model group, and the difference was statistically significant (P<0.05). CYP24A1 in the model group was higher than that in the control, MTX, VD, SMT-M and SMT-H groups, while CYP27B1 and VDR in the model group were lower than those in the control, MTX, VD, SMT-M and SMT-H groups, MPO was higher than that of the control, MTX, VD, SMT-L, SMT-M and SMT-H groups, and NE was higher than the control, VD, SMT-M and SMT-H groups, and the difference was statistically significant (P<0.05) . Conclusion Simiao decoction may inhibit the formation of NETs by regulating the vitamin D system, thereby treating RA.
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