Relation between incorporation of triglyceride fatty acids and heparin-released lipoprotein lipase from adipose tissue slices*

Abstract

The mechanism of the incorporation of plasma triglyceride fatty acids (TGFA) into adipose tissue was investigated. Slices of adipose tissue from rabbits in different nutritional states were incubated under various conditions with plasma very low-density lipoproteins (d <1.006), in which the TGFA had been biologically labeled with palmitate-1-C14. Lipoprotein lipase activity, released into a heparin-containing medium, was assayed in the same tissues. The results show that the incorporation of TGFA into the slices is dependent on the nutritional state of the animal and is positively correlated with the lipoprotein lipase activity released from the tissue under the influence of heparin, which in turn probably correlates with the total lipoprotein lipase activity of the tissue.