Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats

Abstract

Background/Aims: This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats. Materials and Methods: A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3, 5-HT4, and 5-HT7receptors in the colonic tissue was detected using immunohistochemistry, real-time PCR and Western blotting techniques. Results: There is no significant difference on the protein expression of 5-HT3receptor between the normal group and the chronic diarrhea model group. The mRNA expression of 5-HT3receptor in the chronic diarrhea model group was significantly lower than that in the normal group (n = 10; P< 0.01). The protein and mRNA expression of 5-HT4receptor in the chronic diarrhea model group were significantly higher than those in the normal group (n = 10; P< 0.05, P< 0.01). On the contrary, the protein and mRNA expressions of 5-HT7receptor in the chronic diarrhea model group were significantly decreased compared with the normal group (n = 10; P< 0.01, P< 0.01). Conclusions: The results suggested the receptors of 5-HT4and 5-HT7may be involved in inducing diarrhea by lactose diet.

Keywords

• 5-HT3
• 5-HT4
• and 5-HT7 receptors
• chronic diarrhea
• colonic tissue
• lactose