Chemokine receptor CCR3 is important for migration of mast cells in neurofibroma

Abstract

Background: Neurofibroma consists of abundant extracellular matrix and many types of cells, including Schwann cells (SCs), mast cells (MCs), fibroblasts and endothelial cells. As SCs have been found to be the cell of origin for neurofibroma, how MCs may migrate into the tumor has not been fully clarified. Given that chemokine receptor CCR3 is found predominantly expressed by differentiated MCs, we postulated that CCR3 may play a role in the homing of MCs to neurofibroma. The goal of this study is to investigate the possible involvement of chemokine receptor CCR3 in the migration of MCs to the neurofibroma. Methods: Expressional and functional assays for CCR3 and its ligands were performed on MCs and SCs. Results: By real-time quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, we found one of the CCR3 ligand, CCL7 was highly expressed by murine SC cell line SW10, and also moderately expressed by MCs. In serial chemotaxis assays, MCs were found specifically responsive to CCL7 and also condition medium from SW10 cells, indicating SCs may attract MCs by CCR3-mediated cell migration. Conclusion: The interaction of CCR3 and CCL7 may play important roles for MC migration toward SC in the neurofibroma.

Keywords

• Chemokine
• CCR3 receptor
• Mast cells
• Neurofibroma