ASSOCIATIONS OF FUNCTIONAL PARAMETERS OF ERYTHROCYTES WITH THE STRUCTURE OF THEIR MEMBRANES IN MEN OF NOVOSIBIRSK WITH ATHEROGENIC DYSLIPIDEMIA AND ALCOHOLIC LIVER DISEASE
M. V. Kruchinina,
M. V. Parulikova,
S. A. Kurilovich,
A. A. Gromov,
V. M. Generalov,
V. A. Richter,
D. V. Semenov,
S. V. Morozov,
A. S. Sokolova,
N. F. Salakhutdinov,
M. V. Shashkov,
V. N. Sidelnikov,
V. N. Kruchinin
Affiliations
M. V. Kruchinina
Institute of Internal and Preventive Medicine - Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian Federation
M. V. Parulikova
Institute of Internal and Preventive Medicine - Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian Federation
S. A. Kurilovich
Institute of Internal and Preventive Medicine - Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian Federation
A. A. Gromov
Institute of Internal and Preventive Medicine - Branch of Federal Research Institute of Cytology and Genetics of SB RAS Russian Federation
V. M. Generalov
State Research Center of Virology and Biotechnology «Vector», Federal Service for Surveillance on Consumer Rights Protection and Human Well-being Russian Federation
V. A. Richter
Institute of Bioorganic Chemistry and Fundamental Medicine of SB RAS Russian Federation
D. V. Semenov
Institute of Bioorganic Chemistry and Fundamental Medicine of SB RAS Russian Federation
S. V. Morozov
Institute of Organic Chemistry n.a. N.N. Vorozhtsov of SB RAS Russian Federation
A. S. Sokolova
Institute of Organic Chemistry n.a. N.N. Vorozhtsov of SB RAS Russian Federation
N. F. Salakhutdinov
Institute of Organic Chemistry n.a. N.N. Vorozhtsov of SB RAS Russian Federation
M. V. Shashkov
Institute of Catalysis n.a. G.K. Boreskov of SB RAS Russian Federation
V. N. Sidelnikov
Institute of Catalysis n.a. G.K. Boreskov of SB RAS Russian Federation
V. N. Kruchinin
Institute of Semiconductor Physics n.a. A.V. Rzhanov of SB RAS Russian Federation
Under supervision there were 151 men (age from 35 till 60 years) with diffuse hepatic diseases (97 with chronic hepatitis, 33 with liver cirrhosis, 21 with fatty liver disease). Alcohol liver disease (ALD) was determined in 66 men. Biochemical and instrumental studies, ultrasonic examination of liver, spleen as well as portal vessels were performed for determination stadia of disease. Moreover, liver biopsy was performed in 19 patients for verification of diagnosis. To all men were carried out inspections of structure-functional erythrocyte characteristics by methods of dielectrophoresis, thin-layer, gas chromatography, GC/MS system. Is experimentally established: rigidity, viscosity, electric conductivity of a membrane of erythrocytes, indexes of agregation and destruction are increased, but the amplitude of deformation of erythrocytes, polarizability are decreased in ALD. An abnormally high content of cholesterol, saturated fatty acids and low levels of total lipids, phospholipids, triglyceridies, ethers of cholesterol, unsaturated fatty acids were found in red cell membranes from patients with ALD. The molar ratio cholesterol/phospholipids was increased at the expense of decreased level of total phospholipids (fractions of phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and sphingomieline). In patients with fatty liver disease, unidirectional changes in the spectrum of fatty acids in the membranes of erythrocytes and serum (increased level of saturated with decreasing unsaturated) were detected, associations with the manifestations of atherogenic dyslipidemia were established. Correlations between amplitude of deformation of erythrocytes, rigidity, viscosity, electric conductivity of a membrane of erythrocytes, polarizability, index of destruction and levels of total cholesterol, lysophospholipids, polyunsaturated fatty acids in red cells can be used as additional characteristics with a view of early diagnostics of alcoholic liver disease.
