Effect of Germinated Black Rice Krisna Extract on Fasting Blood Glucose and Body Weight in Diabetes Mellitus Rats

Abstract

Background: Diabetes mellitus (DM) is a non-communicable disease that is generally associated with fasting blood glucose (FBG) level of >126 mg/dL and weight loss. Germinated black rice krisna (GBRK) extract contains phytochemicals with antioxidant and anti-diabetic properties. The purpose of this study was to assess the impact of GBRK extract on FBG level and body weight (BW) in rat model of DM.Methods: In an in vivo pre- and post-test study 36 male Wistar rats with BW design, of 150-200 g were enrolled and divided into 6 groups, including control (C), negative control (NC), GBRK 535 mg/kg BW (P1), GBRK 1070 mg/kg. BW (P2), and GBRK 2140 mg/kg BW (P3). Diabetes was induced using streptozotocin (STZ) and nicotinamide (NA) and the intervention period was 14 days. Measurement of FBG level was by quantitative enzymatic colorimetric test of Glucose Oxidase Phenol 4-Aminoantipyrine (GOD-PAP) and body weight was determined by a digital scale.Results: The administration of GBRK extract in P1, P2, and P3 groups could significantly reduce the FBG (p <0.01). GBRK in P1, P2, and P3 groups could also significantly improve the body weight to normal state too (p <0.01).Conclusion: GBRK extract at doses of 535, 1070, and 2140 mg/kg BW for 14 days was demonstrated to improve metabolic disorders in diabetes by reducing FBG level and improving the BW to the normal state.

Keywords

• germinated black rice
• diabetes mellitus
• blood glucose
• body weight
• antioxidant