Scientia Pharmaceutica (Sep 2019)

Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids

  • Julia Eichsteininger,
  • Kerstin Kirisits,
  • Claudia Smöch,
  • Christa Stadlbauer,
  • Chi Huu Nguyen,
  • Walter Jäger,
  • Ali Özmen,
  • Gerhard Ecker,
  • Georg Krupitza,
  • Liselotte Krenn

DOI
https://doi.org/10.3390/scipharm87030023
Journal volume & issue
Vol. 87, no. 3
p. 23

Abstract

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We investigated the effect of 21 flavonoids in a three-dimensional in vitro system for their ability to inhibit gap formation by MCF-7 breast cancer spheroids in monolayers of lymphendothelial cells. Different representatives of the classes of flavones, flavonols, and flavanones were tested in the circular chemorepellent-induced defects (CCID)-assay. Bay11-7082, a known inhibitor of CCID formation served as the positive control. This study provides the first comparison of the potential of flavonoids to suppress features influencing the intravasation of MCF-7 breast cancer cells aggregates through the lymph endothelial barrier. The most significant effects were seen after incubation with the flavones luteolin, chrysin, and apigenin. Additional hydroxylation or methoxylation in positions 6 or 8, as expected, resulted in decreased activity. The tested flavanones remained without or low efficacy.

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