Therapeutic Study of Cinnamic Acid Derivative for Oxidative Stress Ablation: The Computational and Experimental Answers
Oluwafemi Adeleke Ojo,
Akingbolabo Daniel Ogunlakin,
Rotdelmwa Filibis Maimako,
Gideon Ampoma Gyebi,
Christopher Busayo Olowosoke,
Odunayo Anthonia Taiwo,
Tobiloba Christiana Elebiyo,
David Adeniyi,
Bolaji David,
Matthew Iyobhebhe,
Juliana Bunmi Adetunji,
Damilare IyinKristi Ayokunle,
Adebola Busola Ojo,
Ramzi A. Mothana,
Abdullah R. Alanzi
Affiliations
Oluwafemi Adeleke Ojo
Good Health and Wellbeing Research Cluster, Bowen University, Iwo 232102, Nigeria
Akingbolabo Daniel Ogunlakin
Good Health and Wellbeing Research Cluster, Bowen University, Iwo 232102, Nigeria
Rotdelmwa Filibis Maimako
Department of Biochemistry, Landmark University, Omu-Aran 251101, Nigeria
Gideon Ampoma Gyebi
Natural Products and Structural (Bio-Chem)-Informatics Research Laboratory (NpsBC-RI), Department of Biochemistry, Bingham University, Karu 961105, Nigeria
Christopher Busayo Olowosoke
Department of Biotechnology, Federal University of Technology, PMB 704 Futa Road, Akure 340252, Nigeria
Odunayo Anthonia Taiwo
Department of Biochemistry, Chrisland University, Abeokuta 110118, Nigeria
Tobiloba Christiana Elebiyo
Department of Biochemistry, Landmark University, Omu-Aran 251101, Nigeria
David Adeniyi
Good Health and Wellbeing Research Cluster, Bowen University, Iwo 232102, Nigeria
Bolaji David
Good Health and Wellbeing Research Cluster, Bowen University, Iwo 232102, Nigeria
Matthew Iyobhebhe
Department of Biochemistry, Landmark University, Omu-Aran 251101, Nigeria
Juliana Bunmi Adetunji
Department of Biochemistry, Osun State University, Osogbo 232106, Nigeria
Damilare IyinKristi Ayokunle
Department of Pure and Applied Biology, Bowen University, Iwo 232101, Nigeria
Adebola Busola Ojo
Department of Biochemistry, Ekiti State University, Ado-Ekiti 362103, Nigeria
Ramzi A. Mothana
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Abdullah R. Alanzi
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
This study aimed to examine the therapeutic activity of the cinnamic acid derivative KAD-7 (N′-(2,4-dichlorobenzylidene)-3-(4-methoxyphenyl) acrylohydrazide) on Fe2+-induced oxidative hepatic injury via experimental and computational models. In addition, the role of ATPase and ectonucleoside triphosphate diphosphohydrolase (ENTPDase) in the coordination of cellular signals is speculated upon to proffer suitable therapeutics for metabolic stress disorder upon their inhibition. While we know little about therapeutics with flexible dual inhibitors for these protein targets, this study was designed to screen KAD-7’s (N′-(2,4-dichlorobenzylidene)-3-(4-methoxyphenyl) acrylohydrazide) inhibitory potential for both protein targets. We induced oxidative hepatic damage via the incubation of hepatic tissue supernatant with 0.1 mM FeSO4 for 30 min at 37 °C. We achieved the treatment by incubating the hepatic tissues with KAD-7 under the same conditions. The catalase (CAT), glutathione (GSH), malondialdehyde (MDA), ATPase, and ENTPDase activity were all measured in the tissues. We predicted how the drug candidate would work against ATPase and ENTPDase targets using molecular methods. When hepatic injury was induced, there was a significant decrease in the levels of the GSH, CAT, and ENTPDase (p p 4 by reducing oxidative stress and purinergic actions. Our studies indicate that KAD-7 could be developed as a therapeutic option since it can flexibly inhibit both ATPase and ENTPDase.