A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft
Masaaki Ito,
Taku Toriumi,
Takahiro Hiratsuka,
Hideto Imura,
Yasunori Akiyama,
Ichinnorov Chimedtseren,
Yoshinori Arai,
Kazuhiro Yamaguchi,
Akihiko Azuma,
Ken-ichiro Hata,
Nagato Natsume,
Masaki Honda
Affiliations
Masaaki Ito
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan
Taku Toriumi
Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, Aichi 464-8650, Japan
Takahiro Hiratsuka
Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan
Hideto Imura
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan
Yasunori Akiyama
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan
Ichinnorov Chimedtseren
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan
Yoshinori Arai
Department of Oral and Maxillofacial Radiology, Nihon University School of Dentistry, Tokyo 101-8310, Japan
Kazuhiro Yamaguchi
Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan
Akihiko Azuma
Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan
Ken-ichiro Hata
Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan
Nagato Natsume
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan
Masaki Honda
Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, Aichi 464-8650, Japan
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation—and could be useful in human alveolar cleft repairs as well.
