Nature Communications (Jan 2020)

Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy

  • Alyssa Sheih,
  • Valentin Voillet,
  • Laïla-Aïcha Hanafi,
  • Hannah A. DeBerg,
  • Masanao Yajima,
  • Reed Hawkins,
  • Vivian Gersuk,
  • Stanley R. Riddell,
  • David G. Maloney,
  • Martin E. Wohlfahrt,
  • Dnyanada Pande,
  • Mark R. Enstrom,
  • Hans-Peter Kiem,
  • Jennifer E. Adair,
  • Raphaël Gottardo,
  • Peter S. Linsley,
  • Cameron J. Turtle

DOI
https://doi.org/10.1038/s41467-019-13880-1
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 13

Abstract

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Understanding factors that impact CAR T cell expansion in the clinic is crucial to improving its therapeutic success. Here the authors document heterogeneity in the clonal dynamics of CAR-T cells by tracking individual clones using the endogenous TCR and integration sites and provide further insights into the role of transcriptional states in clonal kinetics.