Effect of Lactobacillus reuteri cell wall and cytoplasmic extract on stimulating of IL-4 and IFN γ cytokines production

Abstract

Introduction: Lactobacillus bacteria are known as living microorganisms in the stomach, biliary secretions and pancreas. They attach to the epithelial cells and colonize the human intestines. Many probiotics belong to a large group of bacteria called lactic acid bacteria. In according to immunomodulatory effect of probiotics and effect of these bacteria on the effectiveness of immune responses, we proposed the evaluation of Lactobacillus reuteri cell wall and cytoplasmic extract effects on stimulating of IL-4 and IFN γ cytokines production. Material and Methods: For this purpose 20 samples of traditional dairy and pasteurized dairy products were collected. Molecular identification was performed using molecular-specific primers. Bacterial DNA was extracted and identification of bacterial species was determined by genetic sequencing of PCR product. Human heparin blood was then prepared and peripheral blood mononuclear cells were isolated. To evaluate immune stimulation, IL-4 and IFN γ cytokines produced by ELISA were measured. Results: The results of this study showed that from 20 dairy samples, 7 isolates of Lactobacillus reuteri was identified. The immune system for the production of interleukin-4 and IFN γ in the presence of cell wall and cytoplasmic cell extracts of Lactobacillus reuteri was not stimulated. Lactobacillus casei extract compared to other two bacteria has the greatest effect on immune stimulation and increased IL-4 production. Discussion and conclusion: The level of cytokines produced in human monocular cells did not increase after stimulating the immune system. The Lactobacillus reuteri Bacterium will not be used in immunotherapy.

Keywords

• lactobacillus casei
• immunotherapy
• cytokines
• pbmc