Long-Term Tracking of the Effects of Colostrum-Derived <i>Lacticaseibacillus rhamnosus</i> Probio-M9 on Gut Microbiota in Mice with Colitis-Associated Tumorigenesis
Feiyan Zhao,
Keizo Hiraishi,
Xiaodong Li,
Yaopeng Hu,
Daibo Kojima,
Zhihong Sun,
Heping Zhang,
Lin-Hai Kurahara
Affiliations
Feiyan Zhao
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China
Keizo Hiraishi
Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, Japan
Xiaodong Li
Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, Japan
Yaopeng Hu
Department of Physiology, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Fukuoka, Japan
Daibo Kojima
Department of Gastroenterological Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Fukuoka, Japan
Zhihong Sun
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China
Heping Zhang
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China
Lin-Hai Kurahara
Department of Cardiovascular Physiology, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Kagawa, Japan
Intestinal bacteria play important roles in the progression of colitis-associated carcinogenesis. Colostrum-derived Lacticaseibacillus rhamnosus Probio-M9 (Probio-M9) has shown a protective effect in a colitis-associated cancer (CAC) model, but detailed metagenomic analysis had not been performed. Here, we investigated the preventive effects of the probiotic Probio-M9 on CAC-model mice, tracking the microbiota. Feces were obtained at four time points for evaluation of gut microbiota. The effect of Probio-M9 on tight junction protein expression was evaluated in co-cultured Caco-2 cells. Probio-M9 treatment decreased the number of tumors as well as stool consistency score, spleen weight, inflammatory score, and macrophage expression in the CAC model. Probio-M9 accelerated the recovery of the structure, composition, and function of the intestinal microbiota destroyed by azoxymethane (AOM)/dextran sulfate sodium (DSS) by regulating key bacteria (including Lactobacillus murinus, Muribaculaceae bacterium DSM 103720, Muribaculum intestinale, and Lachnospiraceae bacterium A4) and pathways from immediately after administration until the end of the experiment. Probio-M9 co-culture protected against lipopolysaccharide-induced impairment of tight junctions in Caco-2 cells. This study provides valuable insight into the role of Probio-M9 in correcting gut microbiota defects associated with inflammatory bowel disease carcinogenesis and may have clinical application in the treatment of inflammatory carcinogenesis.
