Nature Communications (May 2017)

Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

  • Peer Arts,
  • Jori van der Raadt,
  • Sebastianus H.C. van Gestel,
  • Marloes Steehouwer,
  • Jay Shendure,
  • Alexander Hoischen,
  • Cornelis A. Albers

DOI
https://doi.org/10.1038/ncomms15190
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

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Transcriptome sequencing is a powerful tool for functional analysis of different types of RNA molecules in a wide range of applications. Here the authors use targeted resequencing of cDNA with single-molecule molecular inversion probes as a cost-effective, high-throughput tool for mRNA quantification.