Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)

Jun Ma; Baofeng Feng; Desheng Kong; Jingjing He; Ruiyun Guo; Asiamah Ernest Amponsah; Wei Zhang; Shuhan Zhang; Fei Lv; Yongzhou Song; Aijing Liu; Huixian Cui

Stem Cell Research (Apr 2020)

Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)

Jun Ma,
Baofeng Feng,
Desheng Kong,
Jingjing He,
Ruiyun Guo,
Asiamah Ernest Amponsah,
Wei Zhang,
Shuhan Zhang,
Fei Lv,
Yongzhou Song,
Aijing Liu,
Huixian Cui

Affiliations

Jun Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China; Human Anatomy Department, Hebei Medical University, Hebei 050017, China
Baofeng Feng: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Desheng Kong: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Jingjing He: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Ruiyun Guo: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Asiamah Ernest Amponsah: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Wei Zhang: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Shuhan Zhang: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Fei Lv: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China
Yongzhou Song: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; The Second Hospital of Hebei Medical University, Hebei 050017, China
Aijing Liu: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; The Second Hospital of Hebei Medical University, Hebei 050017, China
Huixian Cui: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei 050017, China; Human Anatomy Department, Hebei Medical University, Hebei 050017, China; Corresponding author at: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei 050017, China.

Journal volume & issue: Vol. 44

Abstract

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Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with the loss of upper motor neurons in the cortex and lower motor neurons in the brain stem and spinal cord regressively. The vast majority of ALS cases have no familial history are apparently sporadic (SALS), making the modeling of SALS essential to the development of ALS therapeutics. Therefore, human induced pluripotent stem cell (iPSC) from peripheral blood mononuclear cells of a 64-year-old SALS patient were produced using a virus-free protocol and characterized using standard validate methods. This generated iPSC line could be useful to reveal SALS mechanisms and screen drug development. Keywords: induced pluripotent stem cell, sporadic amyotrophic lateral sclerosis, reprogramming, differentiation

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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