High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination

Ping Lin; Qinqin Pu; Qun Wu; Chuanmin Zhou; Biao Wang; Jacob Schettler; Zhihan Wang; Shugang Qin; Pan Gao; Rongpeng Li; Guoping Li; Zhenyu Cheng; Lefu Lan; Jianxin Jiang; Min Wu

doi:10.1038/s41467-019-11695-8

Nature Communications (Aug 2019)

High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination

Ping Lin,
Qinqin Pu,
Qun Wu,
Chuanmin Zhou,
Biao Wang,
Jacob Schettler,
Zhihan Wang,
Shugang Qin,
Pan Gao,
Rongpeng Li,
Guoping Li,
Zhenyu Cheng,
Lefu Lan,
Jianxin Jiang,
Min Wu

Affiliations

Ping Lin: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University
Qinqin Pu: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Qun Wu: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Chuanmin Zhou: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Biao Wang: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Jacob Schettler: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Zhihan Wang: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Shugang Qin: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Pan Gao: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota
Rongpeng Li: Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University
Guoping Li: Southwestern Medical University, Pulmonary and Allergy Institute, Affiliated Hospital
Zhenyu Cheng: Department of Microbiology and Immunology, Dalhousie University
Lefu Lan: Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Jianxin Jiang: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University
Min Wu: Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota

DOI: https://doi.org/10.1038/s41467-019-11695-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

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Small non-coding RNAs (sRNA) regulate bacterial functions by finding nucleic acids and proteins. Here the authors identify PhrS sRNA in Pseudomonas as a positive regulator of CRISPR, and show PhrS acts by binding to CRISPR leader, thereby preventing Rho-mediated transcription termination and promoting anti-bacteriophage immunity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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