CryoET structures of immature HIV Gag reveal six-helix bundle

Luiza Mendonça; Dapeng Sun; Jiying Ning; Jiwei Liu; Abhay Kotecha; Mateusz Olek; Thomas Frosio; Xiaofeng Fu; Benjamin A. Himes; Alex B. Kleinpeter; Eric O. Freed; Jing Zhou; Christopher Aiken; Peijun Zhang

doi:10.1038/s42003-021-01999-1

Communications Biology (Apr 2021)

CryoET structures of immature HIV Gag reveal six-helix bundle

Luiza Mendonça,
Dapeng Sun,
Jiying Ning,
Jiwei Liu,
Abhay Kotecha,
Mateusz Olek,
Thomas Frosio,
Xiaofeng Fu,
Benjamin A. Himes,
Alex B. Kleinpeter,
Eric O. Freed,
Jing Zhou,
Christopher Aiken,
Peijun Zhang

Affiliations

Luiza Mendonça: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Dapeng Sun: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Jiying Ning: Department of Structural Biology, University of Pittsburgh School of Medicine
Jiwei Liu: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford
Abhay Kotecha: Thermo Fisher Scientific
Mateusz Olek: Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus
Thomas Frosio: Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus
Xiaofeng Fu: Department of Structural Biology, University of Pittsburgh School of Medicine
Benjamin A. Himes: Department of Structural Biology, University of Pittsburgh School of Medicine
Alex B. Kleinpeter: Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute
Eric O. Freed: Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute
Jing Zhou: Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center
Christopher Aiken: Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center
Peijun Zhang: Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

DOI: https://doi.org/10.1038/s42003-021-01999-1
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 9

Abstract

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Mendonça et al. determine the structures of both in vitro assemblies of the HIV structural precursor protein Gag and Gag viral-like particles (VLPs) to 4.2 Å and 4.5 Å resolutions, using cryo-electron tomography and subtomogram averaging. This study provides insights into the future development of small molecule inhibitors for HIV-1 infection.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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