Genome-wide recombination map construction from single sperm sequencing in cattle
Liu Yang,
Yahui Gao,
Mingxun Li,
Ki-Eun Park,
Shuli Liu,
Xiaolong Kang,
Mei Liu,
Adam Oswalt,
Lingzhao Fang,
Bhanu P. Telugu,
Charles G. Sattler,
Cong-jun Li,
John B. Cole,
Eyal Seroussi,
Lingyang Xu,
Lv Yang,
Yang Zhou,
Li Li,
Hongping Zhang,
Benjamin D. Rosen,
Curtis P. Van Tassell,
Li Ma,
George E. Liu
Affiliations
Liu Yang
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Yahui Gao
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Mingxun Li
College of Animal Science and Technology, Yangzhou University
Ki-Eun Park
Department of Animal and Avian Sciences, University of Maryland
Shuli Liu
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Xiaolong Kang
College of Agriculture, Ningxia University
Mei Liu
Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University
Adam Oswalt
Select Sires Inc
Lingzhao Fang
The Roslin Institute, Royal (Dick) School of Veterinary Studies, The University of Edinburgh
Bhanu P. Telugu
Department of Animal and Avian Sciences, University of Maryland
Charles G. Sattler
Select Sires Inc
Cong-jun Li
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
John B. Cole
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Eyal Seroussi
Agricultural Research Organization (ARO), Volcani Center, Institute of Animal Science
Lingyang Xu
Innovation Team of Cattle Genetic Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
Lv Yang
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University
Yang Zhou
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University
Li Li
College of Animal Science and Technology, Sichuan Agricultural University
Hongping Zhang
College of Animal Science and Technology, Sichuan Agricultural University
Benjamin D. Rosen
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Curtis P. Van Tassell
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Li Ma
Department of Animal and Avian Sciences, University of Maryland
George E. Liu
Animal Genomics and Improvement Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, USDA
Abstract Background Meiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle. Results To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle. Conclusions To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.
