Determination of the Prevalence of Gardnerella vaginalis, Trichomonas vaginalis and Athophobium vaginalis in Shahid Akbarabadi Hospital of Tehran with Multiplex PCR

Abstract

Background: More than 30 bacterial, viral, and parasitic pathogens have the potential for transfusion (sexually transmitted), an infectious group called sexually transmitted infections (STIs). Atofobium vagina, Gardnerella vaginalis are one of the most common causes of the disease. T. vaginalis is also the most common protozoa among vaginosis causing agents. This study aimed to determine the prevalence of these factors in conjunction with the multiplex PCR method. Methods: In this study, among 320 women who referred to Shahid Akbarabadi Hospital in Tehran, 70 samples of people with symptoms of vaginosis who had not taken antibiotics at least one week before the visit were selected. The results of the Amsel criteria and Nugent score tests indicated only 27 cases as bacterial vaginosis. After DNA extraction from the specimens, a multiplex PCR was performed on the samples. Results: The results of the multiplex PCR showed that in 27 cases, 14 cases of bacterial vaginosis only in terms of A. vaginalis, 21 cases were only G. vaginalis and 10 were positive for both bacteria and in the case of T. vaginalis only 1 positive sample was observed among all samples of vaginosis, which was also positive in the A. vaginalis. Conclusion: The results of this study showed that multiplex PCR could be of great help in the diagnosis of bacterial vaginitis diseases.

Keywords

• A. vaginalis
• G. vaginalis
• T. vaginalis
• multiplex PCR