Effects of Contamination Detergent (Las) On The Expression Of iNOS In Kidney Common Carp (Cyprinus carpio L.)

Abstract

This research aims to know the expression of iNOS in the kidney of fish contamination of LAS (linear alkylbenzene sulfonat). Expression of iNOS observations of renal cells cranial common carp by using immunohistochemical methods show that distribution in the cytoplasma and iNOS cell nucleus. Expression of iNOS in the head kidney tissue distribution carp tubules and other networks is uneven. Expression of iNOS on a network of tubules occurs in cells on the outside. On other network distribution unevenly iNOS expression. Expression of iNOS in the head kidney tissues of common carp found in the control group and treatment group LAS contamination during 48 hours of observation. Expression of iNOS in the control group or normal ranges from 4.25-5.5 % of cells, treatment A (0,01 mg/L LAS) 10.5-15.75 % cell, treatment B (0,02 mg/L LAS) 9.75-22.25 % cell, treatment C (0,03 mg/L LAS) 10.75-21.00 %, treatment D (0,04 mg/L LAS) 14.5-18.00 % cell and treatment E (0,05 mg/L LAS) 13.00-18.00 % cell. Standard deviation on the control ranges from 2,08 up 3.4 while in group treatment ranged 1 to 1.25

Keywords

• LAS, expression of iNOS, Cyprinus carpio L.