Host Range Limitation of a Pseudorecombinant Virus Produced by Two Distinct Bipartite Geminiviruses

Abstract

The production of pseudorecombinant viruses following exchange of cloned genomic components of the distinct geminiviruses Sida golden mosaic virus (SiGMV-Co) and Abutilon mosaic virus (AbMV) has been investigated. The pseudorecombinant virus produced by DNA A of AbMV and DNA B of SiGMV-Co was infectious in various host plants, although showing a limited host range compared with the two parental viruses. The reciprocal exchange of genomic components did not result in an infectious pseudorecombinant virus. However, in leaf disks SiGMV-Co DNA A was able to trans-replicate the heterogenomic AbMV DNA B component.

Keywords

• pseudorecombination
• trans-replication