Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation
Emilie Viennois,
Anika Tahsin,
Didier Merlin
Affiliations
Emilie Viennois
Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, USA
Anika Tahsin
Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, USA
Didier Merlin
Institute for Biomedical Sciences, Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA, USAVeterans Affairs Medical Center, Decatur, GA, USA
We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.
