Journal of Synchrotron Radiation (Nov 2022)
AMX – the highly automated macromolecular crystallography (17-ID-1) beamline at the NSLS-II
- Dieter K. Schneider,
- Alexei S. Soares,
- Edwin O. Lazo,
- Dale F. Kreitler,
- Kun Qian,
- Martin R. Fuchs,
- Dileep K. Bhogadi,
- Steve Antonelli,
- Stuart S. Myers,
- Bruno S. Martins,
- John M. Skinner,
- Jun Aishima,
- Herbert J. Bernstein,
- Thomas Langdon,
- John Lara,
- Robert Petkus,
- Matt Cowan,
- Leonid Flaks,
- Thomas Smith,
- Grace Shea-McCarthy,
- Mourad Idir,
- Lei Huang,
- Oleg Chubar,
- Robert M. Sweet,
- Lonny E. Berman,
- Sean McSweeney,
- Jean Jakoncic
Affiliations
- Dieter K. Schneider
- New York Structural Biology Center, New York, New York, USA
- Alexei S. Soares
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Edwin O. Lazo
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Dale F. Kreitler
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Kun Qian
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Martin R. Fuchs
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Dileep K. Bhogadi
- LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA
- Steve Antonelli
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Stuart S. Myers
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Bruno S. Martins
- Azure Data, Microsoft (United States), Atlanta, Georgia, USA
- John M. Skinner
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Jun Aishima
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Herbert J. Bernstein
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Thomas Langdon
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- John Lara
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Robert Petkus
- Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
- Matt Cowan
- CSI, Brookhaven National Laboratory, Upton, New York, USA
- Leonid Flaks
- Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA
- Thomas Smith
- Physics Department, Brookhaven National Laboratory, Upton, New York, USA
- Grace Shea-McCarthy
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Mourad Idir
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Lei Huang
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Oleg Chubar
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Robert M. Sweet
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Lonny E. Berman
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Sean McSweeney
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- Jean Jakoncic
- NSLS-II, Brookhaven National Laboratory, Upton, New York, USA
- DOI
- https://doi.org/10.1107/S1600577522009377
- Journal volume & issue
-
Vol. 29,
no. 6
pp. 1480 – 1494
Abstract
The highly automated macromolecular crystallography beamline AMX/17-ID-1 is an undulator-based high-intensity (>5 × 1012 photons s−1), micro-focus (7 µm × 5 µm), low-divergence (1 mrad × 0.35 mrad) energy-tunable (5–18 keV) beamline at the NSLS-II, Brookhaven National Laboratory, Upton, NY, USA. It is one of the three life science beamlines constructed by the NIH under the ABBIX project and it shares sector 17-ID with the FMX beamline, the frontier micro-focus macromolecular crystallography beamline. AMX saw first light in March 2016 and started general user operation in February 2017. At AMX, emphasis has been placed on high throughput, high capacity, and automation to enable data collection from the most challenging projects using an intense micro-focus beam. Here, the current state and capabilities of the beamline are reported, and the different macromolecular crystallography experiments that are routinely performed at AMX/17-ID-1 as well as some plans for the near future are presented.
Keywords
- macromolecular crystallography
- automation
- beamline
- synchrotron source
- high throughput
- micro-beam
- real-time feedback
