Porin from Marine Bacterium <i>Marinomonas primoryensis</i> KMM 3633<sup>T</sup>: Isolation, Physico-Chemical Properties, and Functional Activity
Olga D. Novikova,
Valentina A. Khomenko,
Natalia Yu. Kim,
Galina N. Likhatskaya,
Lyudmila A. Romanenko,
Ekaterina I. Aksenova,
Marina S. Kunda,
Natalia N. Ryzhova,
Olga Yu. Portnyagina,
Tamara F. Solov’eva,
Olga L. Voronina
Affiliations
Olga D. Novikova
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Valentina A. Khomenko
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Natalia Yu. Kim
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Galina N. Likhatskaya
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Lyudmila A. Romanenko
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Ekaterina I. Aksenova
N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Str., 18, Moscow 123098, Russia
Marina S. Kunda
N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Str., 18, Moscow 123098, Russia
Natalia N. Ryzhova
N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Str., 18, Moscow 123098, Russia
Olga Yu. Portnyagina
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Tamara F. Solov’eva
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russia
Olga L. Voronina
N. F. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Str., 18, Moscow 123098, Russia
Marinomonas primoryensis KMM 3633T, extreme living marine bacterium was isolated from a sample of coastal sea ice in the Amursky Bay near Vladivostok, Russia. The goal of our investigation is to study outer membrane channels determining cell permeability. Porin from M. primoryensis KMM 3633T (MpOmp) has been isolated and characterized. Amino acid analysis and whole genome sequencing were the sources of amino acid data of porin, identified as Porin_4 according to the conservative domain searching. The amino acid composition of MpOmp distinguished by high content of acidic amino acids and low content of sulfur-containing amino acids, but there are no tryptophan residues in its molecule. The native MpOmp existed as a trimer. The reconstitution of MpOmp into black lipid membranes demonstrated its ability to form ion channels whose conductivity depends on the electrolyte concentration. The spatial structure of MpOmp had features typical for the classical gram-negative porins. However, the oligomeric structure of isolated MpOmp was distinguished by very low stability: heat-modified monomer was already observed at 30 °C. The data obtained suggest the stabilizing role of lipids in the natural membrane of marine bacteria in the formation of the oligomeric structure of porin.