The study on neuroprotective mechanism of water extract of Fomitopsis Pinicola on mesencephala dopaminergic neurons induced by MPP+

TMR Modern Herbal Medicine. 2019;2(2):74-82 DOI 10.12032/TMRmhm2017A46


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Journal Title: TMR Modern Herbal Medicine

ISSN: 2537-9798 (Online)

Publisher: TMR publishing group

LCC Subject Category: Medicine

Country of publisher: China

Language of fulltext: English

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Shanshan Guo (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.)
Wolf Dieter Rausch (Institute for Medical Biochemistry, University for Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria.)


Double blind peer review

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Time From Submission to Publication: 4 weeks


Abstract | Full Text

Objective: To observe the neuroprotective mechanism of water extract of Fomitopsis Pinicola on MPP+ induced apoptosis of mesencephala dopaminergic cells in vitro. Methods: The antioxidant activity of fungi was determined by FRAP method. The anti-inflammatory activity of the fungi was detected by LPS-induced NO release method. Mesencephalic dopaminergic neurons were labeled by TH staining to observe the survival of THir neurons. Results: In the anti-oxidant activity assay, the Trolox equivalent anti-oxidant capacity (TEAC) of water extract of Fomitopsis Pinicola was determined to be (165.80±7.13)μmol Trolox/g extract. Water extracts of Fomitopsis Pinicola treatment(100,50,25,12.5μg/ml) decreased NO formation significantly. MPP+ induced significant chromatin condensation in the nuclei of mesencephala dopaminergic neurons with nuclear lysis,the mitochondrial membrane potential decreased remarkably, and ROS production increased significantly. Compared with the MPP+ control group, the morphological changes of cell nuclei after apoptosis was reversed by water extract of Fomitopsis Pinicola. Water extract of Fomitopsis Pinicola treatment(50,25,12.5μg/ml) dramatically increased relative mitochondrial membrane potential compared with MPP+ control respectively. Compared with the MPP+ control, water extract of Fomitopsis Pinicola treatment (50, 25μg/ml) significantly decreased relative ROS formation respectively. Conclusions:Water extract of Fomitopsis Pinicola showed significant neuroprotective effect on mesencephala dopaminergic cells induced by MPP+. The water extract of Fomitopsis Pinicola showed antioxidant and anti-inflammatory activities. The mechanism of neuroprotective effect of water extract of Fomitopsis Pinicola may be related to inhibitory on mitochondrial oxidative stress.