Retinoic acid triazole promotes neurotrophin-mediated cell growth and proliferation in nerve cells

Abstract

In the present study, fluorophenyl retinoic acid triazole, a retinoic acid derivative having more bioavailability compared to the parent retinoic acid was investigated against cell growth and proliferation. The results indicated that fluorophenyl retinoic acid triazole leads to a significant expression of TRK mRNA in time a dependent manner in NB1643 cells. There were about 10-, 14-, and 21-fold induction of expression after 1, 2, and 3 days of treatment, respectively. The results revealed that TRK and TRKB were induced at 6 and 12 hours afterthe treatment respectively and the expression of TRKB was maximum at day 3. Fluorophenyl retinoic acid triazole exerts neurite outgrowth activity by inducing TRKB expression, which is a receptor for brain-derived neurotrophic factor and a protein-tyrosine kinase. Fluorophenyl retinoic acid triazole accumulation generates many small vesiclesin the cell which assembles at the neck of each small prominence and lead to significant morphological changes after 24 hours of treatment.

Keywords

• neurotrophin
• neurite growth activity
• brain derived nerve growth factor
• bioavailability