In vitro conservation of potato (Solanum tuberosum L.) accessions in the Gene Bank of Republic of Srpska

Abstract

Two accessions of potato (Glamočki and Rogatički) were used for the in vitro culture introduction in the Gene Bank of the Republic of Srpska. As explants for introduction are used germs of potato tubers. After surface sterilization, explants were inoculated on MS medium with 3% sucrose without hormones. Development of the explants was followed by five weeks and than were done selecting of survived explants. Number of survived explants was 70% for accessions of Glamočki potato, and 40% for Rogatički potato. Developed explants were used for next passage to period of 5 weeks and after that by micropropagation procedure transfered to MS medium with 3% sucrose and hormones BAP and IBA. After 5 weeks mesuring of lenth of shoots and determining of tubers number was performed. It was found that accessions of Glamočki potato had an average length of 12,24 cm of shoot while accessions of Rogatički potato had an average length 7,92. Average number of tubers per accession Glamočki potato had 1,81 while Rogatički had 1,21.

Keywords

• ms medium
• glamočki
• rogatički
• explant