Use of Magnetic Nanoparticles Fe3O4 in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase

Abstract

In this study, magnetic nanoparticles Fe3O4synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were obtained Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP. The activity of the synthesized biocatalysts was estimated spectrophotometrically in the oxidation reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The optimal conditions for this reaction were selected (pH 6.0, temperature 45 °C). It has been determined that biocatalysts Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP slightly decreased their activity during 5 consecutive experiments, by 19 and 15 %.