Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012
Atsushi Yamanaka,
Mami Matsuda,
Tamaki Okabayashi,
Pannamthip Pitaksajjakul,
Pongrama Ramasoota,
Kyoko Saito,
Masayoshi Fukasawa,
Kentaro Hanada,
Tomokazu Matsuura,
Masamichi Muramatsu,
Tatsuo Shioda,
Ryosuke Suzuki
Affiliations
Atsushi Yamanaka
Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Mami Matsuda
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan
Tamaki Okabayashi
Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
Pannamthip Pitaksajjakul
Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Pongrama Ramasoota
Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Kyoko Saito
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Masayoshi Fukasawa
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Kentaro Hanada
Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Tomokazu Matsuura
Department of Laboratory Medicine, The Jikei University School of Medicine, Tokyo, Japan
Neutralization tests are the most reliable assay for flavivirus antibody detection; however, these assays are not suitable for high-throughput processing due to their time-consuming and labor-intensive nature. In this study, we developed single-round infectious particles (SRIPs) with a luciferase gene for dengue virus types 1 to 4, Japanese encephalitis virus, and Zika virus for use in a safe, high-throughput neutralization assay.
