Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012

Atsushi Yamanaka; Mami Matsuda; Tamaki Okabayashi; Pannamthip Pitaksajjakul; Pongrama Ramasoota; Kyoko Saito; Masayoshi Fukasawa; Kentaro Hanada; Tomokazu Matsuura; Masamichi Muramatsu; Tatsuo Shioda; Ryosuke Suzuki

doi:10.1128/mSphere.00339-21

mSphere (Aug 2021)

Seroprevalence of Flavivirus Neutralizing Antibodies in Thailand by High-Throughput Neutralization Assay: Endemic Circulation of Zika Virus before 2012

Atsushi Yamanaka,
Mami Matsuda,
Tamaki Okabayashi,
Pannamthip Pitaksajjakul,
Pongrama Ramasoota,
Kyoko Saito,
Masayoshi Fukasawa,
Kentaro Hanada,
Tomokazu Matsuura,
Masamichi Muramatsu,
Tatsuo Shioda,
Ryosuke Suzuki

Affiliations

Atsushi Yamanaka: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Mami Matsuda: Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan
Tamaki Okabayashi: Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan
Pannamthip Pitaksajjakul: Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Pongrama Ramasoota: Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Kyoko Saito: Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Masayoshi Fukasawa: Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Kentaro Hanada: Department of Biochemistry and Cell Biology, National Institute of Infectious Diseases, Tokyo, Japan
Tomokazu Matsuura: Department of Laboratory Medicine, The Jikei University School of Medicine, Tokyo, Japan
Masamichi Muramatsu: ORCiD; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan
Tatsuo Shioda: Mahidol-Osaka Center for Infectious Diseases, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
Ryosuke Suzuki: ORCiD; Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

DOI: https://doi.org/10.1128/mSphere.00339-21
Journal volume & issue: Vol. 6, no. 4

Abstract

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Neutralization tests are the most reliable assay for flavivirus antibody detection; however, these assays are not suitable for high-throughput processing due to their time-consuming and labor-intensive nature. In this study, we developed single-round infectious particles (SRIPs) with a luciferase gene for dengue virus types 1 to 4, Japanese encephalitis virus, and Zika virus for use in a safe, high-throughput neutralization assay.

Published in mSphere

ISSN: 2379-5042 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/msphere

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