Frontiers in Cellular and Infection Microbiology (Apr 2023)
Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease
- Chenguang Niu,
- Chenguang Niu,
- Chenguang Niu,
- Chenguang Niu,
- Chenguang Niu,
- Ye Tu,
- Ye Tu,
- Ye Tu,
- Ye Tu,
- Ye Tu,
- Qiaoqiao Jin,
- Qiaoqiao Jin,
- Qiaoqiao Jin,
- Qiaoqiao Jin,
- Qiaoqiao Jin,
- Zhanyi Chen,
- Zhanyi Chen,
- Zhanyi Chen,
- Zhanyi Chen,
- Zhanyi Chen,
- Keyong Yuan,
- Keyong Yuan,
- Keyong Yuan,
- Keyong Yuan,
- Keyong Yuan,
- Min Wang,
- Min Wang,
- Pengfei Zhang,
- Pengfei Zhang,
- Pengfei Zhang,
- Pengfei Zhang,
- Pengfei Zhang,
- Junyuan Luo,
- Junyuan Luo,
- Junyuan Luo,
- Junyuan Luo,
- Junyuan Luo,
- Hao Li,
- Hao Li,
- Hao Li,
- Hao Li,
- Hao Li,
- Yueyi Yang,
- Yueyi Yang,
- Yueyi Yang,
- Yueyi Yang,
- Yueyi Yang,
- Xiaoyu Liu,
- Xiaoyu Liu,
- Xiaoyu Liu,
- Xiaoyu Liu,
- Xiaoyu Liu,
- Mengying Mao,
- Mengying Mao,
- Mengying Mao,
- Mengying Mao,
- Mengying Mao,
- Ting Dong,
- Ting Dong,
- Ting Dong,
- Ting Dong,
- Ting Dong,
- Wenduo Tan,
- Wenduo Tan,
- Wenduo Tan,
- Wenduo Tan,
- Wenduo Tan,
- Xuchen Hu,
- Xuchen Hu,
- Xuchen Hu,
- Xuchen Hu,
- Xuchen Hu,
- Yihuai Pan,
- Yihuai Pan,
- Lili Hou,
- Rui Ma,
- Rui Ma,
- Rui Ma,
- Rui Ma,
- Rui Ma,
- Zhengwei Huang,
- Zhengwei Huang,
- Zhengwei Huang,
- Zhengwei Huang,
- Zhengwei Huang
Affiliations
- Chenguang Niu
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Chenguang Niu
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Chenguang Niu
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Chenguang Niu
- National Center for Stomatology, Shanghai, China
- Chenguang Niu
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Ye Tu
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Ye Tu
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Ye Tu
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Ye Tu
- National Center for Stomatology, Shanghai, China
- Ye Tu
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Qiaoqiao Jin
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Qiaoqiao Jin
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Qiaoqiao Jin
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Qiaoqiao Jin
- National Center for Stomatology, Shanghai, China
- Qiaoqiao Jin
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Zhanyi Chen
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Zhanyi Chen
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Zhanyi Chen
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Zhanyi Chen
- National Center for Stomatology, Shanghai, China
- Zhanyi Chen
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Keyong Yuan
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Keyong Yuan
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Keyong Yuan
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Keyong Yuan
- National Center for Stomatology, Shanghai, China
- Keyong Yuan
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Min Wang
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Min Wang
- Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Pengfei Zhang
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Pengfei Zhang
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Pengfei Zhang
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Pengfei Zhang
- National Center for Stomatology, Shanghai, China
- Pengfei Zhang
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Junyuan Luo
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Junyuan Luo
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Junyuan Luo
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Junyuan Luo
- National Center for Stomatology, Shanghai, China
- Junyuan Luo
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Hao Li
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Hao Li
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Hao Li
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Hao Li
- National Center for Stomatology, Shanghai, China
- Hao Li
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Yueyi Yang
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Yueyi Yang
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Yueyi Yang
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Yueyi Yang
- National Center for Stomatology, Shanghai, China
- Yueyi Yang
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Xiaoyu Liu
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xiaoyu Liu
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Xiaoyu Liu
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Xiaoyu Liu
- National Center for Stomatology, Shanghai, China
- Xiaoyu Liu
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Mengying Mao
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Mengying Mao
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Mengying Mao
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Mengying Mao
- National Center for Stomatology, Shanghai, China
- Mengying Mao
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Ting Dong
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Ting Dong
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Ting Dong
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Ting Dong
- National Center for Stomatology, Shanghai, China
- Ting Dong
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Wenduo Tan
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Wenduo Tan
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Wenduo Tan
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Wenduo Tan
- National Center for Stomatology, Shanghai, China
- Wenduo Tan
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Xuchen Hu
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xuchen Hu
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Xuchen Hu
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Xuchen Hu
- National Center for Stomatology, Shanghai, China
- Xuchen Hu
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Yihuai Pan
- Institute of Stomatology, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Yihuai Pan
- Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China
- Lili Hou
- Department of Nursing, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China
- Rui Ma
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Rui Ma
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Rui Ma
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Rui Ma
- National Center for Stomatology, Shanghai, China
- Rui Ma
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- Zhengwei Huang
- Department of Endodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Zhengwei Huang
- College of Stomatology, Shanghai Jiao Tong University, Shanghai, China
- Zhengwei Huang
- National Clinical Research Center for Oral Diseases, Shanghai, China
- Zhengwei Huang
- National Center for Stomatology, Shanghai, China
- Zhengwei Huang
- Shanghai Key Laboratory of Stomatology, Shanghai, China
- DOI
- https://doi.org/10.3389/fcimb.2023.1157368
- Journal volume & issue
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Vol. 13
Abstract
Metabolic dysfunction-associated fatty liver disease (MAFLD) is a phenotype of liver diseases associated with metabolic syndrome. The pathogenesis MAFLD remains unclear. The liver maintains is located near the intestine and is physiologically interdependent with the intestine via metabolic exchange and microbial transmission, underpinning the recently proposed “oral-gut-liver axis” concept. However, little is known about the roles of commensal fungi in the disease development. This study aimed to characterize the alterations of oral and gut mycobiota and their roles in MAFLD. Twenty-one MAFLD participants and 20 healthy controls were enrolled. Metagenomics analyses of saliva, supragingival plaques, and feces revealed significant alterations in the gut fungal composition of MAFLD patients. Although no statistical difference was evident in the oral mycobiome diversity within MAFLD and healthy group, significantly decreased diversities were observed in fecal samples of MAFLD patients. The relative abundance of one salivary species, five supragingival species, and seven fecal species was significantly altered in MAFLD patients. Twenty-two salivary, 23 supragingival, and 22 fecal species were associated with clinical parameters. Concerning the different functions of fungal species, pathways involved in metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and carbon metabolism were abundant both in the oral and gut mycobiomes. Moreover, different fungal contributions in core functions were observed between MAFLD patients and the healthy controls, especially in the supragingival plaque and fecal samples. Finally, correlation analysis between oral/gut mycobiome and clinical parameters identified correlations of certain fungal species in both oral and gut niches. Particularly, Mucor ambiguus, which was abundant both in saliva and feces, was positively correlated with body mass index, total cholesterol, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase, providing evidence of a possible “oral-gut-liver” axis. The findings illustrate the potential correlation between core mycobiome and the development of MAFLD and could propose potential therapeutic strategies.
Keywords
- metabolic dysfunction-associated fatty liver disease
- oral mycobiome
- gut mycobiome
- oral-gut axis
- metagenomics
