The human neuroprotective placental protein composition suppressing tinnitus and restoring auditory brainstem response in a rodent model of sodium salicylate-induced ototoxicity
Mohammad Farhadi,
Ali Gorji,
Marjan Mirsalehi,
Marcus Müller,
Alexander Borisovich Poletaev,
Fereidoun Mahboudi,
Abdoreza Asadpour,
Mohammad Ebrahimi,
Mohaddeseh Beiranvand,
Mohaddeseh Dehghani Khaftari,
Zeinab Akbarnejad,
Saeid Mahmoudian
Affiliations
Mohammad Farhadi
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Ali Gorji
Epilepsy Research Center, Department of Neurosurgery Westfälische Wilhelms-Universitat Münster, Münster, Germany; Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Shefa Neuroscience Research Center Khatam Alanbia Hospital, Tehran, Iran
Marjan Mirsalehi
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Marcus Müller
Translational Hearing Research, Tübingen Hearing Research Center, Department of Otolaryngology, Head & Neck Surgery, University of Tübingen, 72076, Tübingen, Germany
Alexander Borisovich Poletaev
Clinical and Research Center of Children Psycho-Neurology, Moscow, Russian Federation; Medical Research Centre “Immunculus”, Moscow, Russian Federation
Fereidoun Mahboudi
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abdoreza Asadpour
Intelligent Systems Research Center, Ulster University, Magee Campus, Derry∼Londonderry, Northern Ireland, UK
Mohammad Ebrahimi
The Research Center for New Technologies in Life Sciences Engineering, Tehran University, Tehran, Iran
Mohaddeseh Beiranvand
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Mohaddeseh Dehghani Khaftari
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Zeinab Akbarnejad
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Corresponding author. Auditory Cognitive Neuroscience Lab, ENT and Head and Neck Research Centre, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Neyiayesh St, Sattar Khan Ave., P.O. Box 14455-3641445613131, Tehran, Iran.
Saeid Mahmoudian
ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Corresponding author. Auditory Cognitive Neuroscience Lab, ENT and Head and Neck Research Centre, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Neyiayesh St, Sattar Khan Ave., P.O. Box 14455-3641445613131, Tehran, Iran.
The effect of neuroprotective placental protein composition (NPPC) on the suppression of tinnitus and the restoration of the auditory brainstem response (ABR) characteristics was explored in tinnitus-induced rats. The animals were placed into two groups: (1) the study group, rats received sodium salicylate (SS) at the dose of 200 mg/kg twice a day for two weeks, and then 0.4 mg of the NPPC per day, between the 14th and 28th days, (2) the placebo group, rats received saline for two weeks, and then the NPPC alone between the 14th and 28th days. The gap pre-pulse inhibition of the acoustic startle (GPIAS), the pre-pulse inhibition (PPI), and the ABR assessments were performed on animals in both groups three times (baseline, day 14, and 28). The GPIAS value declined after 14 consecutive days of the SS injection, while NPPC treatment augmented the GPIAS score in the study group on the 28th day. The PPI outcomes revealed no significant changes, indicating hearing preservation after the SS and NPPC administrations. Moreover, some changes in ABR characteristics were observed following SS injection, including (1) higher ABR thresholds, (2) lowered waves I and II amplitudes at the frequencies of 6, 12, and 24 kHz and wave III at the 12 kHz, (3) elevated amplitude ratios, and (4) prolongation in brainstem transmission time (BTT). All the mentioned variables returned to their normal values after applying the NPPC. The NPPC use could exert positive therapeutic effects on the tinnitus-induced rats and improve their ABR parameters.
