Nature Communications (Nov 2017)

Break-induced replication promotes formation of lethal joint molecules dissolved by Srs2

  • Rajula Elango,
  • Ziwei Sheng,
  • Jessica Jackson,
  • Jenna DeCata,
  • Younis Ibrahim,
  • Nhung T. Pham,
  • Diana H. Liang,
  • Cynthia J. Sakofsky,
  • Alessandro Vindigni,
  • Kirill S. Lobachev,
  • Grzegorz Ira,
  • Anna Malkova

DOI
https://doi.org/10.1038/s41467-017-01987-2
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 13

Abstract

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Break-induced replication (BIR) is a double-strand break repair pathway that can lead to genomic instability. Here the authors show that the absence of Srs2 helicase during BIR leads to uncontrolled binding of Rad51 to single-stranded DNA, which promotes the formation of toxic intermediates that need to be resolved by Mus81 or Yen1.