Accurate detection of m6A RNA modifications in native RNA sequences

Huanle Liu; Oguzhan Begik; Morghan C. Lucas; Jose Miguel Ramirez; Christopher E. Mason; David Wiener; Schraga Schwartz; John S. Mattick; Martin A. Smith; Eva Maria Novoa

doi:10.1038/s41467-019-11713-9

Nature Communications (Sep 2019)

Accurate detection of m6A RNA modifications in native RNA sequences

Huanle Liu,
Oguzhan Begik,
Morghan C. Lucas,
Jose Miguel Ramirez,
Christopher E. Mason,
David Wiener,
Schraga Schwartz,
John S. Mattick,
Martin A. Smith,
Eva Maria Novoa

Affiliations

Huanle Liu: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Oguzhan Begik: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Morghan C. Lucas: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Jose Miguel Ramirez: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Christopher E. Mason: Department of Physiology and Biophysics, Weill Cornell Medicine
David Wiener: Department of Molecular Genetics, Weizmann Institute of Science
Schraga Schwartz: Department of Molecular Genetics, Weizmann Institute of Science
John S. Mattick: Department of Neuroscience, Garvan Institute of Medical Research
Martin A. Smith: St-Vincent’s Clinical School, UNSW Sydney
Eva Maria Novoa: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology

DOI: https://doi.org/10.1038/s41467-019-11713-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 9

Abstract

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We currently lack generic methods to map RNA modifications across the entire transcriptome. Here, the authors demonstrate that m6A RNA modifications can be detected with high accuracy using nanopore direct RNA sequencing.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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