Selected amino acids uptake by microorganisms in soil of differently managed mountain meadows

Abstract

This work was aimed at determination of the effect of 13 years abandonment of previously long-term mown mountain meadows on uptake of L-glutamic acid (14CO2H[14CH2]2[NH2]14CO2H) and L-alanine (14CH314CH[NH2]14CO2H) by microbial community of Ap horizon (3–13 cm). The study plots has been located near to the experimental stand “Bílý Kříž“ which is located in the Moravian-Silesian Beskids Mountains (N 49°30’17”, E 18°32’28”), on a slope with an elevation of 825–860 m a. s. l. and southeast orientation, and soil classified as an Oxyaquic Hapludalf. The study was performed to test method for measurement of 14C-labelled amino acids uptake by soil microbial community and to increase knowledge on particular processes of N-transformation in soil of these ecosystems. The results obtained in this work showed that 13 years abandonment of mountain meadow did not significantly (P > 0.05) influence rate of glutamic acid or alanine uptake by soil microbial community. Further research including determination of amino acids use by soil microbial biomass with expression of their partitioning between production of new microbial biomass and energy metabolism is necessary.

Keywords

• soil
• amino acids
• uptake
• glutamic acid
• alanine
• meadow