Genome-Wide Identification of Banana <em>Csl</em> Gene Family and Their Different Responses to Low Temperature between Chilling-Sensitive and Tolerant Cultivars
Weina Yuan,
Jing Liu,
Tomáš Takáč,
Houbin Chen,
Xiaoquan Li,
Jian Meng,
Yehuan Tan,
Tong Ning,
Zhenting He,
Ganjun Yi,
Chunxiang Xu
Affiliations
Weina Yuan
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Jing Liu
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Tomáš Takáč
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University Olomouc, 783 75 Olomouc, Czech Republic
Houbin Chen
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Xiaoquan Li
Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
Jian Meng
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Yehuan Tan
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Tong Ning
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Zhenting He
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Ganjun Yi
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Chunxiang Xu
Department of Pomology, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
The cell wall plays an important role in responses to various stresses. The cellulose synthase-like gene (Csl) family has been reported to be involved in the biosynthesis of the hemicellulose backbone. However, little information is available on their involvement in plant tolerance to low-temperature (LT) stress. In this study, a total of 42 Csls were identified in Musa acuminata and clustered into six subfamilies (CslA, CslC, CslD, CslE, CslG, and CslH) according to phylogenetic relationships. The genomic features of MaCsl genes were characterized to identify gene structures, conserved motifs and the distribution among chromosomes. A phylogenetic tree was constructed to show the diversity in these genes. Different changes in hemicellulose content between chilling-tolerant and chilling-sensitive banana cultivars under LT were observed, suggesting that certain types of hemicellulose are involved in LT stress tolerance in banana. Thus, the expression patterns of MaCsl genes in both cultivars after LT treatment were investigated by RNA sequencing (RNA-Seq) technique followed by quantitative real-time PCR (qPCR) validation. The results indicated that MaCslA4/12, MaCslD4 and MaCslE2 are promising candidates determining the chilling tolerance of banana. Our results provide the first genome-wide characterization of the MaCsls in banana, and open the door for further functional studies.
